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Multidimensional Nonlinear Femtosecond Spectroscopy of Biological Molecules

Craig Lincoln

Centre for Atom Optics and Ultrafast Spectroscopy, Swinburne University of Technology

3:30pm Friday 29th April, Seminar Room AR103, Graduate Research Centre

Multidimensional nonlinear femtosecond spectroscopy offers many advantages over conventional absorption based measurements in determining the coupling strength and time scales for optical dephasing, energy transfer and intra-molecular vibrational motion in addition to population lifetimes. In this talk we will introduce a new multidimensional technique based on spectrally resolved two-colour three-pulse photon echo spectroscopy in the visible region. We begin with a theoretical treatment to compare the spectrally resolved measurement to existing nonlinear techniques and study the signal dependence on different laser frequencies and pulse widths. We then apply the spectrally resolved measurement to the laser dye rhodamine 101 to characterise the spectrally resolved measurement of a real system. Finally, we will describe a study of a photolytic dissociation of the carbonyl complexes of biologically important heme proteins myoglobin and hemoglobin. We estimate the time scale of the rapid dissociation process (<20 fs) and determine the existence of an intermediate excited state.

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