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Human Frontiers - Superresolution

This project aims to develop high spatial resolution imaging techniques based on photoactivatable fluorescent proteins and localization algorithms, beyond the diffraction limit of conventional imaging techniques. The higher resolution available with this technique allows researchers to view cell interactions with greater detail.


Project Overview:

Group:
Aim:
This project aims to develop high spatial resolution imaging techniques based on photoactivatable fluorescent proteins and localization algorithms, beyond the diffraction limit of conventional imaging techniques.
Members:

Research Overview:

Achievements for 2008
  • Development of image processing software to identify and localize single fluorescent particles with a high precision, and render a superresolved image based on single particle statistics.
  • Awarded FABLS Support Scheme for emerging research projects AU$5380.71.
  • Lab exchange to Betzig Lab, Howard Hughes Medical Institute, Janelia Farm Research Campus for building a photoactivatable localization microscope at CMP.
Aims for 2009
  • Completion of the PALM image processing software and development of a graphical user interface.
  • Development of image processing software for characterisation of blinking statistics of single nano-particles.


Figure: Single quantum dot imaging, where the first five images in a series of 100 images are displayed (Top row). The blinking characteristics of two selected single quantum dots are illustrated in the middle and bottom rows, respectively.

Project Leader:
Professor
Sarah Russell

ph: +61 3 9656 3727
srussell@swin.edu.au

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