Skip to Content

Focussing, Aberration Correction and Enhanced Optical Sectioning in Microscopy

Professor Tony Wilson

Department of Engineering Science, Oxford University, UK

3:30 pm Monday, 1 February 2010, EN101 (Ground Floor, EN Building), Hawthorn.

There are many factors that influence the design of a confocal microscope.  Among these crucially is the method of achieving optical sectioning or confocal operation. High resolution imaging of thick specimens is also often detrimentally affected by specimen-induced aberrations that cause a loss in signal strength together with reduced resolution. Aberrations can be corrected using adaptive optics, where a deformable mirror, for example, introduces equal but opposite aberrations into the optical path.  We will discuss the design of a wave front sensorless adaptive system. We will describe such a system where the refocusing may be achieved remotely from the specimen at high speed without introducing spherical aberration that is common to other optical refocusing systems.

Back to 2010 programme

Top