TY - JOUR T1 - Differential regulation of osteogenic differentiation of stem cells on surface roughness gradients JO - Biomaterials VL - 35 IS - 33 SP - 9023 EP - 9032 PY - 2014/11// T2 - AU - Faia-Torres, Ana B. AU - Guimond-Lischer, Stefanie AU - Rottmar, Markus AU - Charnley, Mirren AU - Goren, Tolga AU - Maniura-Weber, Katharina AU - Spencer, Nicholas D. AU - Reis, Rui L. AU - Textor, Marcus AU - Neves, Nuno M. SN - 0142-9612 DO - http://dx.doi.org/10.1016/j.biomaterials.2014.07.015 UR - http://www.sciencedirect.com/science/article/pii/S0142961214008096 KW - Surface roughness KW - Mesenchymal stem cell KW - Polycaprolactone KW - Osteogenesis AB - Abstract Tissue engineering using scaffold-cell constructs holds the potential to develop functional strategies to regenerate bone. The interface of orthopedic implants with the host tissues is of great importance for its later performance. Thus, the optimization of the implant surface in a way that could stimulate osteogenic differentiation of mesenchymal stem cells (MSCs) is of significant therapeutic interest. The effect of surface roughness of polycaprolactone (PCL) on the osteogenic differentiation of human bone-marrow MSCs was investigated. We prepared surface roughness gradients of average roughness (Ra) varying from the sub-micron to the micrometer range (∼0.5–4.7 μm), and mean distance between peaks (RSm) gradually varying from ∼214 μm to 33 μm. We analyzed the degree of cytoskeleton spreading, expression of alkaline phosphatase, collagen type 1 and mineralization. The response of cells to roughness divided the gradient into three groups of elicited stem cell behavior: 1) faster osteogenic commitment and strongest osteogenic expression; 2) slower osteogenic commitment but strong osteogenic expression, and 3) similar or inferior osteogenic potential in comparison to the control material. The stem-cell modulation by specific PCL roughness surfaces highlights the potential for creating effective solutions for orthopedic applications featuring a clinically relevant biodegradable material. ER -