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Assoc. Prof. Andrew Clayton

Associate Professor of Biophotonics




Contact Details:

Office: EN126d
Phone: +61 3 9214 5719
Fax: +61 3 9214 5435
Email: aclayton@swin.edu.au

Mail To:

Swinburne University of Technology
Centre for Micro-Photonics (H74)
John Street
PO Box 218
Hawthorn, Victoria , 3122
Australia

Research Interests
 1. Cell Surface Receptors

Receptors provide the first line of communication between cells and their external environment. Receptor malfunction has been implicated in diseases such as cancer.


Our recent research has unveiled a new mechanism of receptor activation on the cell surface that involves the formation of higher-order polymeric complexes. We wish to establish the generality of this phenomenon and its implication to cell signaling and therapeutic intervention. The project draws on several areas, molecular biology, cell biology, multidimensional microscopy and systems biology.

2. New optical tools for biology


Development and application of FRET, FLIM, polarization and correlation imaging methods to measure protein-protein interactions and dynamics at the single cell and single molecule level.

3. Peptide-membrane interactions

The interaction of macromolecules with biological surfaces such as membranes represents a fundamental question and is important in areas such as the development of new antimicrobial peptides.


In collaboration with A/Prof. Michelle Gee at the University of Melbourne we aim to understand the molecular dynamics and molecular states produced by antimicrobial peptides with artificial membranes and cells using surface-selective spectroscopy and microscopic techniques.
Membership of Professional Organisations
 Australian Biophysics Society

US Biophysical Society
Qualifications
 PhD, University of Melbourne, 1996
Areas of Expertise and Research
 Andrew Clayton undertook his Ph.D. studies in physical chemistry at the University of Melbourne (Prof. Ken Ghiggino) then spent time as ARC research fellow in the Biochemistry and Molecular Biology Department studying biophysics (Prof. Bill Sawyer). He pursued further postdoctoral studies at the Max-Planck Institute for Biophysical Chemistry in Goettingen (Dr. Thomas Jovin) as Human Frontier Science Program Fellow before returning to Melbourne initially as HFSP fellow (Prof. Tony Burgess) then NHMRC RD Wright Fellow and Head of the Cell Biophysics Laboratory at the Ludwig Institute for Cancer Research.

His present research focuses on understanding the wiring diagram of living cells by measuring macromolecular interactions and dynamics with multidimensional microscopy. His research achievements have been recognized with several awards including Young Fluorescence Investigator 2007 from the US Biophysical Society.
Professional Committees and Activities
 Victorian State Representative of Australian Biophysics Society
Latest Publications
 
17.   Inhibiting EGFR Clustering and Cell Proliferation with Gold Nanoparticles
Chiara Paviolo, James W. M. Chon and Andrew H. A. Clayton
Small 2014 IN PRESS; doi: 10.1002/smll.201402701
Journal Link | Swinburne Research | | RIS Export
     
16.   Polarization of excitation light influences molecule counting in single-molecule localization microscopy
Ye Chen, Han Lin, Mandy J. Ludford-Menting, Andrew H. Clayton, Min Gu and Sarah M. Russell
Histochem Cell Biol 2014 IN PRESS; doi: 10.1007/s00418-014-1267-1
Journal Link | Swinburne Research | | RIS Export
     
15.   Recruitment of the Adaptor Protein Grb2 to EGFR Tetramers
Noga Kozer, Dipak Barua, Christine Henderson, Edouard C. Nice, Antony W. Burgess, William S. Hlavacek and Andrew H. A. Clayton
Biochemistry 53 (16), 2594-2604 (2014); doi: 10.1021/bi500182x
Journal Link | Swinburne Research | | RIS Export
     
14.   Taking Care of Bystander FRET in a Crowded Cell Membrane Environment
Andrew H. A. Clayton and Amitabha Chattopadhyay
Biophysical Journal 106 (6), 1227-1228 (2014); doi: 10.1016/j.bpj.2014.02.004
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13.   Characterization of optical polarization converters made by femtosecond laser writing
Christiaan J. de Jong, Alireza Lajevardipour, Mindaugas Gecevičius, Martynas Beresna, Gediminas Seniutinas, Gediminas Gervinskas, Ričardas Buividas, Peter G. Kazansky, Yves Bellouard, Andrew H. A. Clayton and Saulius Juodkazis
Proc. SPIE 8923, (2013); doi: 10.1117/12.2033928
Journal Link | Swinburne Research | | RIS Export
     
12.   Long time-scale interaction dynamics between a model antimicrobial peptide and giant unilamellar vesicles
Matthew G. Burton, Qi M. Huang, Mohammed A. Hossain, John D. Wade, Andrew Harry A. Clayton and Michelle L Gee
Langmuir 2013 IN PRESS; doi: 10.1021/la403083m
Journal Link | Swinburne Research | | RIS Export
     
11.   Ultra-pure, water-dispersed Au nanoparticles produced by femtosecond laser ablation and fragmentation
Reda Kubiliūtė, Ksenia A Maximova, Alireza Lajevardipour, Jiawey Yong, Jennifer S Hartley, Abu SM Mohsin, Pierre Blandin, James WM Chon, Marc Sentis, Paul R Stoddart, Andrei Kabashin, Ričardas Rotomskis, Andrew HA Clayton and Saulius Juodkazis
Int. J. Nanomedicine 8, 2601- 2611 (2013); doi: 10.2147/IJN.S44163
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10.   Temperature measurement in the microscopic regime: a comparison between fluorescence lifetime- and intensity-based methods
C. Paviolo, A.H.A. Clayton, S.L. Macarthur and P.R. Stoddart
J Microscopy 250 (3), 179-188 (2013); doi: 10.1111/jmi.12033
Journal Link | Swinburne Research | | RIS Export
     
9.   Exploring higher-order EGFR oligomerisation and phosphorylation—a combined experimental and theoretical approach
Noga Kozer, Dipak Barua, Suzanne Orchard, Eduoard C. Nice, Antony W. Burgess, William S. Hlavacek and Andrew H. A. Clayton
Mol. BioSyst. 2013 IN PRESS; doi: 10.1039/C3MB70073A
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8.   Imaging the action of antimicrobial peptides on living bacterial cells
Michelle L. Gee, Matthew Burton, Alistair Grevis-James, Mohammed Akhter Hossain, Sally McArthur, Enzo A. Palombo, John D. Wade and Andrew H. A. Clayton
Sci. Rep. 3, (2013); doi: 10.1038/srep01557
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7.   The Effect of Translational Motion on FLIM Measurements-Single Particle Phasor-FLIM
Alireza Lajevardipour and Andrew H. A. Clayton
J Fluoresc 23 (4), 671-679 (2013); doi: 10.1007/s10895-013-1174-1
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6.   Aggregation Distributions on Cells Determined by Photobleaching Image Correlation Spectroscopy
Giuseppe D. Ciccotosto, Noga Kozer, Timothy T. Y. Chow, James W. M. Chon and Andrew H. A. Clayton
Biophys J 104 (2), 1-9 (2013); doi: 10.1016/j.bpj.2013.01.009
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5.   Targeting of a Conformationally Exposed, Tumor-Specific Epitope of EGFR as a Strategy for Cancer Therapy
Hui K. Gan, Antony W. Burgess, Andrew H. A. Clayton and Andrew M. Scott
Cancer Research 72 (12), 2924-30 (2012); doi: 10.1158/0008-5472.CAN-11-3898
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4.   Slow Insertion Kinetics during Interaction of a Model Antimicrobial Peptide with Unilamellar Phospholipid Vesicles
Zubaidah Ningsih, Mohammed Akhter Hossain, John D. Wade, Andrew H. A. Clayton and Michelle L. Gee
Langmuir 28 (4), 2217-2224 (2012); doi: 10.1021/la203770j
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3.   Conformational Dynamics in a Truncated Epidermal Growth Factor Receptor Ectodomain
Noga Kozer, Julie Rothacker, Antony W. Burgess, Edouard C. Nice and Andrew H. A. Clayton
Biochemistry 50 (23), 5130-5139 (2011); doi: 10.1021/bi200095w
Journal Link | Swinburne Research | | RIS Export