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Multidimensional Nonlinear Femtosecond Spectroscopy of Biological Molecules
Craig Lincoln
Centre for Atom Optics and Ultrafast Spectroscopy, Swinburne University of Technology
3:30pm Friday 29th April, Seminar Room AR103, Graduate Research Centre
Multidimensional nonlinear femtosecond spectroscopy offers many advantages over conventional absorption
based measurements in determining the coupling strength and time scales for optical dephasing, energy transfer and intra-molecular
vibrational motion in addition to population lifetimes. In this talk we will introduce a new multidimensional technique based on
spectrally resolved two-colour three-pulse photon echo spectroscopy in the visible region. We begin with a theoretical treatment
to compare the spectrally resolved measurement to existing nonlinear techniques and study the signal dependence on different
laser frequencies and pulse widths. We then apply the spectrally resolved measurement to the laser dye rhodamine 101 to
characterise the spectrally resolved measurement of a real system. Finally, we will describe a study of a photolytic dissociation
of the carbonyl complexes of biologically important heme proteins myoglobin and hemoglobin. We estimate the time scale of the
rapid dissociation process (<20 fs) and determine the existence of an intermediate excited state.
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